Bone Marrow Aspirate & Trephine Biopsy

Bone marrow aspirate and trephine biopsy is an important diagnostic tool in the diagnosis of many blood disorders. It is a simple, safe and relatively painless procedure which can be performed as out patient. Bone marrow is usually advised in case of un explained anaemias, marrow infiltration such as leukemias and lymphomas, pyrexia of unknown origin etc.

Interpretation
Slides of bone marrow aspirate and trephine are examined by consultant hematologist along with slides of peripheral blood film. This finally helps the reviewer to arrive at a conclusion. If required special stains are also done to support the diagnosis.

Prerequisites
No special instruction required.

Schedule
This test is done through out the week.

Sample Collection
Procedure is performed under aseptic measures skin, subcutaneous and periosteum sites selected for puncture are infiltrated with local anesthetic. Bone marrow aspirate is then passed on perpendicularly into the iliac crest then the stilette is removed followed by suction of marrow contents into the syringe. Bone marrow trephine is performed using ISLAM needle. It is also penetrated perpendicularly through rotating motion. After fixing the needle into the underlying bone, stilette is removed and needle is further penetrated core of the bone is then obtained and touch imprints are made on slides.​